pCMV6-AC-HA Mammalian Expression Vector from OriGene Technologies

Product Specs
ItempCMV6-AC-HA Mammalian Expression Vector
CompanyOriGene Technologies
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPS100004
Quantity10 µg
TypeMammalian Expression Vectors
Selectable MarkerAmpicillin
Conjugate/Tag/LabelHA
ResistanceNeomycin

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OriGene Technologies

9620 Medical Center Drive # 200

Rockville, Maryland 20850

United States

Phone: 1-888-267-4436 (U.S. only) / 301-340-3188
301-340-9254

Company Profile

Website: http://www.origene.com

(1) MICU1 regulates mitochondrial cristae structure and function independently of the mitochondrial Ca 2+ uniporter channelScience SignalingApril 25, 2023Dhanendra Tomar, Manfred Thomas, Joanne F. Garbincius, Devin W. Kolmetzky, Oniel Salik, Pooja Jadiya, Suresh K. Joseph, April C. Carpenter, György Hajnóczky, John W. Elrodusing the SgfI-MluI restriction sites and inserted into the same sites in pCMV6-AC-HA vector (Origene # PS100004). Flag-tagged Mouse MICU1 (NM_144822) mutants; EMREmut KKKKR101-105QQQQQ; EF1mut D233A, E244A; EF2mut

(2) Malat1 deficiency prevents neonatal heart regeneration by inducing cardiomyocyte binucleationJCI InsightMarch 8, 2023Galip S. Aslan, Nicolas Jaé, Yosif Manavski, Youssef Fouani, Mariana Shumliakivska, Lisa Kettenhausen, Luisa Kirchhof, Stefan Günther, Ariane Fischer, Guillermo Luxán, et al.designed by OriGene Technologies using the pCMV6-AC-HA mammalian expression vector as backbone (OriGene, PS100004). ORF sequences of both plasmids are listed in Supplemental Table 2. Cells were seeded 24 hours before

(3) Glucocorticoid Receptor β Isoform Predominates in the Human Dysplastic Brain Region and Is Modulated by Age, Sex, and Antiseizure MedicationInternational Journal of Molecular SciencesApril 29, 2022Rosemary Westcott, Natalie Chung, Arnab Ghosh, Lisa Ferguson, William Bingaman, Imad M. Najm, Chaitali Ghosh(OriGene Technologies, Rockville, MD, USA) cloned in a pCMV6-AC-HA vector (OriGene Technologies, cat. PS100004). To achieve this transfection, 5 µg of HA-GRβ DNA was mixed in serum-free Dulbecco’s modified eagle

(4) Deleterious Rare Mutations of GLI1 Dysregulate Sonic Hedgehog Signaling in Human Congenital Heart DiseaseFrontiers in cardiovascular medicineJanuary 1, 2022Peng R, Li B, Chen S, Shi Z, Yu L, Gao Y, Yang X, Lu L, Wang H.enzyme sites. GLI2 was subcloned into SgfI/NotI restriction enzyme sites of pCMV6-AC-HA (Origene, #PS100004). All plasmid were verified by Sanger sequencing. We then performed the site-directed mutagenesis using

(5) DNA Polymerase ι Interacts with Both the TRAF-like and UBL1-2 Domains of USP7Journal of molecular biologyJanuary 22, 2021Ashton NW, Valles GJ, Jaiswal N, Bezsonova I, Woodgate R.

(6) Protein S-glutathionylation stimulate adipogenesis by stabilizing C/EBPβ in 3T3L1 cellsFASEB journal : official publication of the Federation of American Societies for Experimental BiologyApril 1, 2020Watanabe Y, Watanabe K, Fujioka D, Nakamura K, Nakamura T, Uematsu M, Bachschmid MM, Matsui R, Kugiyama K.plasmid (MR227563), Myc -FLAG-tagged mouse PIAS1 plasmid (MR209770), and C-terminal HA-tag vector (PS100004) were from Origene (Rockville, MD, USA). OxiSelect™ Intracellular ROS Assay Kit was from Cell Biolabs

(7) TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) InteractionFrontiers in cell and developmental biologyJanuary 1, 2020Li B, Niswander LA.into SgfI/MluI restriction enzyme sites of pCMV6-AC-DDK/Myc/HA/GFP/RFP (Origene, #PS100005/ PS100003/ PS100004/ PS100010/ PS100034), respectively, according to the need of specific C-terminal tag. The FL TMEM132A

(8) Von Willebrand factor A domain-containing protein 8 (VWA8) localizes to the matrix side of the inner mitochondrial membraneBiochemical and biophysical research communicationsJanuary 1, 2020Luo M, Ma W, Sand Z, Finlayson J, Wang T, Brinton RD, Willis WT, Mandarino LJ.green fluorescent protein (GFP), ATG-TCCGGA-eGFP, was inserted into a plasmid, pCMV6-AC-HA (Origene #PS100004), to generate the pCMV-SG-eGFP expression construct. Within the cDNA fragment were the following: ATG

(9) MAP4K3/GLK Promotes Lung Cancer Metastasis by Phosphorylating and Activating IQGAP1Cancer researchOctober 1, 2019Chuang HC, Chang CC, Teng CF, Hsueh CH, Chiu LL, Hsu PM, Lee MC, Hsu CP, Chen YR, Liu YC, et al.

(10) C-terminal α Domain of p63 Binds to p300 to Coactivate β-CateninNeoplasia (New York, N.Y.)May 1, 2019Katoh I, Maehata Y, Moriishi K, Hata RI, Kurata SI.

(11) DEAD Box Protein 5 Inhibits Liver Tumorigenesis by Stimulating Autophagy via Interaction with p62/SQSTM1Hepatology (Baltimore, Md.)March 1, 2019Zhang H, Zhang Y, Zhu X, Chen C, Zhang C, Xia Y, Zhao Y, Andrisani O, Kong L.constructed in the pCMV- Tag3B. Beclin 1 (BECN1) was constructed in the pCMV6-AC-HA vector (Origene, PS100004) by standard subcloning. P62-Myc and its deletion mutants were gifts from Dr. Ke Li.(34) p62-HA (28027)

(12) A proteasome-resistant fragment of NIK mediates oncogenic NF-κB signaling in schwannomasHuman molecular geneticsFebruary 15, 2019Gehlhausen JR, Hawley E, Wahle BM, He Y, Edwards D, Rhodes SD, Lajiness JD, Staser K, Chen S, Yang X, et al.

(13) GLK-IKKβ signaling induces dimerization and translocation of the AhR-RORγt complex in IL-17A induction and autoimmune diseaseScience advancesSeptember 1, 2018Chuang HC, Tsai CY, Hsueh CH, Tan TH.

(14) EBP50 suppresses the proliferation of MCF-7 human breast cancer cells via promoting Beclin-1/p62-mediated lysosomal degradation of c-MycActa pharmacologica SinicaAugust 1, 2018Liu H, Zhao WL, Wang JP, Xin BM, Shao RG.control. HA-tagged Beclin-1 (Gene Bank ID: NM_003766.3) was constructed in the pCMV6AC-HA vector (Origene, PS100004) by standard subcloning. Myc-tagged EBP50 (Gene Bank ID: NM_004252.4) was created in the pCMV6-AN-Myc

(15) Cyclophilin A Is Overexpressed in Hepatocellular Carcinoma and Is Associated with the Cell CycleAnticancer researchAugust 1, 2017Gong Z, Chi C, Huang X, Chu H, Wang J, Du F, Jiang L, Chen J.

(16) Characterization of the novel protein KIAA0564 (Von Willebrand Domain-containing Protein 8)Biochemical and biophysical research communicationsJune 3, 2017Luo M, Mengos AE, Ma W, Finlayson J, Bustos RZ, Xiao Zhu Y, Shi CX, Stubblefield TM, Willis WT, Mandarino LJ.VWA8b matched NM_173758.3. Mouse VWA8a and VWA8b cDNA were subcloned into pCMV6-AC-HA plasmid (Origene #PS100004) to generate pCMV-mVWA8a-HA and pCMV-mVWA8b-HA. A deletion of the first 34 amino acids in N-terminus

(17) SPHK1 (sphingosine kinase 1) induces epithelial-mesenchymal transition by promoting the autophagy-linked lysosomal degradation of CDH1/E-cadherin in hepatoma cellsAutophagyMay 4, 2017Hong Liu, Yan Ma, Hong-Wei He, Wu-Li Zhao, Rong-Guang Shao(PS100001, Origene) by standard subcloning. HA-tagged BECN1 was constructed in the pCMV6-AC-HA vector (PS100004, Origene) by standard subcloning. Flag-tagged TRAF2 and its mutant (ΔRING) were cloned into the pFLAG-CMV-2

(18) Palmitoylation regulates the intracellular trafficking and stability of c-MetOncotargetMay 31, 2016Coleman DT, Gray AL, Kridel SJ, Cardelli JA.pLenti-MetGFP (Addgene: 37560, Dr. David Rimm) and ligated into the multi-cloning site of pCMV6-AC-HA (OriGene: PS100004) giving a c-terminal HA-tag. Fifteen separate site-directed mutants were engineered (C/A: 561, 584,

(19) Wip1 suppresses ovarian cancer metastasis through the ATM/AKT/Snail mediated signalingOncotargetMay 17, 2016Yin S, Wang P, Yang L, Liu Y, Wang Y, Liu M, Qi Z, Meng J, Shi TY, Yang G, et al.

(20) MIIP accelerates epidermal growth factor receptor protein turnover and attenuates proliferation in non-small cell lung cancerOncotargetFebruary 23, 2016Jing Wen, Jianhua Fu, Yihong Ling, Wei Zhangby OriGene Technologies, Inc. (Rockville, MD) and based on the Precision Shuttle vector pCMV6-AC-HA (PS100004). MIIP and control shRNA plasmids were purchased from Santa Cruz Biotechnology. Plasmid DNA was transfected

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